Teneurin C-Terminal Associated Peptide (TCAP)-3 Increases Metabolic Activity in Zebrafish

Teneurin C-terminal associated peptides (TCAP), bioactive located on the end of teneurin proteins, have been shown to regulate stress axis functions due high conservation between TCAP and corticotropin releasing factor (CRF). Additionally, recent work demonstrated that can increase metabolism in rats via glucose metabolism. These metabolic actions are not well described other organisms, including teleosts. Here we investigated expression a tcap isoform, tcap-3 , potential role TCAP-3 as regulator across zebrafish life-stages. Using pcr-based analyses, appears be independently transcribed, relation teneurin-3 muscle tissue adult zebrafish. Resazurin, respirometry chambers, mitochondrial analyses were used study effects synthetic rainbow trout (rtTCAP-3) larval Overall, activity was enhanced by 48 h rtTCAP-3 treatment larvae (bath immersion) adults (intraperitoneal injections). This uncoupling, respiration proton leak. protected fish from reduced induced low temperatures. Subsequently, increased output subjected accelerated swimming speeds, demonstrating potent regulation during challenges. Collectively, these results demonstrate conserved roles for activator